AKR1C2 Protein (AA 1-323)

Catalog No. ABIN1096529

This Recombinant AKR1C2 protein is produced in Escherichia coli (E. coli).

Quick Overview for AKR1C2 Protein (AA 1-323) (ABIN1096529)

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-323

Purpose: Recombinant Human Aldo-Keto Reductase 1C2/AKR1C2

Sequence: MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEAVKL AIEAGFHHID SAHVYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNH RLLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Characteristics: Recombinant Human Aldo-Keto Reductase Family 1 Member C2/AKR1C2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Tyr323) of Human AKR1C2.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: DDH2

Background: Aldo-Keto Reductase Family 1 Member C2 (AKR1C2) plays a role in concert with the 5-alpha/5-beta-Steroid Reductases to convert Steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-Tetrahydrosteroids. AKR1C2 catalyzes the inactivation of the most potent androgen 5-alpha-Dihydrotestosterone (5-alpha-DHT) to 5-alpha-Androstane-3-alpha, 17-beta-diol (3-alpha-diol).
Alternative Names: Aldo-Keto Reductase Family 1 Member C2, 3-Alpha-HSD3, Chlordecone Reductase Homolog HAKRD, Dihydrodiol Dehydrogenase 2, DD-2, DD2, Dihydrodiol Dehydrogenase/Bile Acid-Binding Protein, DD/BABP, Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase, Type III 3-Al

Molecular Weight: 36.74 kDa

UniProt: P52895

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process