POLB Protein (AA 2-335) (His tag)
Quick Overview for POLB Protein (AA 2-335) (His tag) (ABIN1096541)
Target
See all POLB ProteinsProtein Type
Origin
Source
Purity
-
-
Protein Characteristics
- AA 2-335
-
Purification tag / Conjugate
- This POLB protein is labelled with His tag.
-
Purpose
- Recombinant Human DNA Polymerase β (C-6His)
-
Sequence
- MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSEVEHHH HHH
-
Characteristics
- Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.
-
Sterility
- 0.2 μm filtered
-
Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System HEK-293 CellsConjugate His tagOrigin HumanPrice starts at $12,595.45Expression System Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin HumanPrice starts at $19,692.86Your project requires further customization? Contact us and discover our custom protein solutions
-
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)
custom-made POLB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)custom-made POLB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (GST tag)POLB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His tag)POLB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Polymerase (DNA Directed), beta (POLB) (AA 1-335) protein (His-IF2DI Tag)POLB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Polymerase (DNA Directed), beta (POLB) (AA 2-335) protein (His-IF2DI Tag)POLB Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
-
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50 % Glycerol, pH 7.8.
-
Preservative
- Dithiothreitol (DTT)
-
Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handling Advice
- minimize freeze-thaw cycles
-
Storage
- -20 °C
-
Storage Comment
- Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
-
Expiry Date
- 6 months
-
-
- POLB (Polymerase (DNA Directed), beta (POLB))
-
Alternative Name
- DNA Polymerase beta
-
Background
-
Alternative Names: DNA Polymerase Beta, POLBHuman DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta.
Alternative Names: DNA Polymerase Beta, POLB -
Molecular Weight
- 39.23 kDa
-
UniProt
- P06746
-
Pathways
- DNA Damage Repair
Target
-
