FBP1 Protein (AA 2-338) (His tag)

Catalog No. ABIN1096624

This Recombinant FBP1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FBP1 Protein (AA 2-338) (His tag) (ABIN1096624)

Target: FBP1 (Fructose-1,6-Bisphosphatase 1 (FBP1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-338

Purification tag / Conjugate: This FBP1 protein is labelled with His tag.

Purpose: Recombinant Human Fructose-1,6-Bisphosphatase 1/FBPase 1 (E. coli, C-6His)

Sequence: ADQAPFDTDV NTLTRFVMEE GRKARGTGEL TQLLNSLCTA VKAISSAVRK AGIAHLYGIA GSTNVTGDQV KKLDVLSNDL VMNMLKSSFA TCVLVSEEDK HAIIVEPEKR GKYVVCFDPL DGSSNIDCLV SVGTIFGIYR KKSTDEPSEK DALQPGRNLV AAGYALYGSA TMLVLAMDCG VNCFMLDPAI GEFILVDKDV KIKKKGKIYS LNEGYARDFD PAVTEYIQRK KFPPDNSAPY GARYVGSMVA DVHRTLVYGG IFLYPANKKS PNGKLRLLYE CNPMAYVMEK AGGMATTGKE AVLDVIPTDI HQRAPVILGS PDDVLEFLKV YEKHSAQVEH HHHHH

Characteristics: Recombinant Human Fructose-1,6-Bisphosphatase 1/FBPase 1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Gln338) of Human FBPase 1.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 20 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: FBP1 (Fructose-1,6-Bisphosphatase 1 (FBP1))

Alternative Name: Fructose-1,6-Bisphosphatase 1/FBPase 1

Background: Fructose-1,6-Bisphosphatase 1 (FBPase 1) is a member of the FBPase class 1 family. FBPase 1 is a gluconeogenesis regulatory protein, which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. FBPase 1 can assume an active R-state, or an inactive T-state. FBPase 1 deficiency is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis in newborn infants or young children. FBPase 1 coupled with phosphofructokinase (PFK) is involved in the metabolism of pancreatic islet cells.
Alternative Names: Fructose-1,6-Bisphosphatase 1, FBPase 1, D-Fructose-1,6-Bisphosphate 1-Phosphohydrolase 1, FBP1, FBP

Molecular Weight: 37.89 kDa

UniProt: P09467

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Dicarboxylic Acid Transport