GNMT Protein (AA 1-294) (His tag)

Catalog No. ABIN1096756

Recombinant GNMT protein expressed in Escherichia coli (E. coli).

Quick Overview for GNMT Protein (AA 1-294) (His tag) (ABIN1096756)

Target: GNMT (Glycine N-Methyltransferase (GNMT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-294

Purification tag / Conjugate: This GNMT protein is labelled with His tag.

Purpose: Recombinant Human Glycine N-Methyltransferase/GNMT (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD

Characteristics: Recombinant Human Glycine N-Methyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Asg294 is expressed with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: GNMT (Glycine N-Methyltransferase (GNMT))

Alternative Name: Glycine-N-methyltransferase

Sub Type: Fusionprotein

Background: Alternative Names: Glycine N-Methyltransferase, GNMTGlycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein. GNMT catalyzes the synthesis of N-methylglycine from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. It can affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine, playing an important role in maintaining normal AdoMet levels. GNMT is highly expressed in liver. As a major folate-binding protein, GNMT takes part in the detoxification pathway. Defects in GNMT are the cause of hypermethioninemia. the patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
Alternative Names: Glycine N-Methyltransferase, GNMT

Molecular Weight: 34.9 kDa

UniProt: Q14749

Pathways: Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process