IL-6 Protein (AA 29-212) (His tag)

Catalog No. ABIN1096956

Recombinant IL-6 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-6 Protein (AA 29-212) (His tag) (ABIN1096956)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 29-212

Purification tag / Conjugate: This IL-6 protein is labelled with His tag.

Purpose: Recombinant Human Interleukin-6/IL-6

Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Characteristics: Recombinant Human Interleukin-6/IL-6 is produced by E. coli transformed with a plasmid contains sequence (Pro29-Met212) of Human IL-6 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Comment:

Biological activity: Recombinant IL-6 is fully biologically active when compared to standards.ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of human TF-1 cells.Specific Activity of 5.0 x 107 IU/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 5.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: Il-6

Sub Type: Fusionprotein

Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist, the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin-6 (IL-6) is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
Alternative Names: Interleukin-6, IL-6, B-Cell Stimulatory Factor 2, BSF-2, CTL Differentiation Factor, CDF, Hybridoma Growth Factor, Interferon Beta-2, IFN-Beta-2, IL6, IFNB2

Molecular Weight: 20.9 kDa

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome