PITRM1 Protein (AA 1-124) (His tag)

Catalog No. ABIN1097179

This Recombinant PITRM1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for PITRM1 Protein (AA 1-124) (His tag) (ABIN1097179)

Target: PITRM1 (Pitrilysin Metallopeptidase 1 (PITRM1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-124

Purification tag / Conjugate: This PITRM1 protein is labelled with His tag.

Purpose: Recombinant Human MEK-Binding Protein 1/MP1/MAPKSP1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MADDLKRFLY KKLPSVEGLH AIVVSDRDGV PVIKVANDNA PEHALRPGFL STFALATDQG SKLGLSKNKS IICYYNTYQV VQFNRLPLVV SFIASSSANT GLIVSLEKEL APLFEELRQV VEVS

Characteristics: Recombinant Human Mitogen-Activated Protein Kinase Scaffold Protein 1/MAPKSP1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val124) of Human LAMTOR3 fused with a 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 5 mM DTT, 30 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PITRM1 (Pitrilysin Metallopeptidase 1 (PITRM1))

Alternative Name: MP1

Sub Type: Fusionprotein

Background: Mitogen-Activated Protein Kinase Scaffold Protein 1 (MAPKSP1) was identified as an interacting protein that belongs to the LAMTOR3 family. MAPKSP1 restricted to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. MAPKSP1 interacts with MAP2K1/MEK1 and MAPK2 and enhances the activation of MAPK2, and thus is thought to function as an adaptor to enhance the efficiency of the MAP kinase cascade.
Alternative Names: Ragulator Complex Protein LAMTOR3, Late Endosomal/Lysosomal Adaptor and MAPK and MTOR Activator 3, MEK-Binding Partner 1, Mp1, Mitogen-Activated Protein Kinase Kinase 1-Interacting Protein 1, Mitogen-Activated Protein Kinase Scaffold Protein 1, LAMTOR3, M

Molecular Weight: 15.78 kDa

UniProt: Q9UHA4

Pathways: PI3K-Akt Signaling, SARS-CoV-2 Protein Interactome