Neurotrophin 3 Protein (NTF3) (AA 139-257)

Catalog No. ABIN1097245

This Recombinant Neurotrophin 3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Neurotrophin 3 Protein (NTF3) (AA 139-257) (ABIN1097245)

Target: Neurotrophin 3 (NTF3)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 139-257

Purpose: Recombinant Human Neurotrophin-3/NT3

Sequence: YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT

Characteristics:

Recombinant Human Neurotrophin-3/NT3

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Neurotrophin 3 (NTF3)

Alternative Name: Neurotrophin-3/NT3

Background: Recombinant Human Neurotrophin-3/NT3 produced in E. coli is a single non-glycosylated polypeptide chain containing 119 AAs with a molecular mass of 13.6 kDa.
Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 AA residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 AA residue mature NT3.The AA sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

Molecular Weight: 13.6 kDa

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway