Neurotrophin 3 Protein (NTF3)

Catalog No. ABIN7318822

Recombinant Neurotrophin 3 protein expressed in Escherichia coli (E. coli).

Quick Overview for Neurotrophin 3 Protein (NTF3) (ABIN7318822)

Target: Neurotrophin 3 (NTF3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human Neurotrophin-3/NTF3 Protein

Sequence: Tyr139-Thr 257

Characteristics: Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized Human TrkB (C-6His)(PKSH033579) at 5 μg/mL (100 μL/well) can bind Human NT-3(PKSH032803): Biotinylated by NHS-biotin prior to testing. The EC₅₀ of Human NT-3(PKSH032803) is 15 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Neurotrophin 3 (NTF3)

Alternative Name: Neurotrophin-3/NTF3

Background: HDNF,NGF-2,NT-3,NTF3,Nerve Growth Factor 2,Neurotrophic Factor,Neurotrophin-3,Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

Molecular Weight:

Calculated MW: 13.6 kDa

Observed MW: 14 kDa

Gene ID: 4908

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway