MMP8 Protein (Catalytic Domain)

Catalog No. ABIN1097894

This Recombinant MMP8 protein is expressed in Escherichia coli (E. coli).

Quick Overview for MMP8 Protein (Catalytic Domain) (ABIN1097894)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Purity: > 95% by SDS-PAGE.

Product Details

Protein Characteristics: Catalytic Domain

Sequence: NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YG

Characteristics: Recombinant matrix metalloproteinase-8 (MMP-8, collagenase-2, neurophil collagenase) cloned from human cDNA, expressed in E.coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262)

Purification: Folding state checked by NMR.

Biological Activity Comment: > 80U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Application Details

Application Notes: Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors

Comment:

The enzyme was observed as a single band migrating at a molecular weight of < 20kDa

Restrictions: For Research Use only

Handling

Buffer: 0.2mg/ml in Tris 20mM pH 7.2, CaCl2 10mM, ZnCl2 0.1mM, NaCl 0.3M, Acetohydroxamic Acid (AHA) 0.5M.

Storage: -20 °C/-80 °C

Storage Comment: -80°C. The enzyme is stable at -20°C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.

Target Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP-8

Molecular Weight: 17.7kDa

UniProt: P22894