MMP8 Protein (Catalytic Domain)
Quick Overview for MMP8 Protein (Catalytic Domain) (ABIN1097894)
Target
See all MMP8 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Catalytic Domain
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Sequence
- NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YG
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Characteristics
- Recombinant matrix metalloproteinase-8 (MMP-8, collagenase-2, neurophil collagenase) cloned from human cDNA, expressed in E.coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262)
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Purification
- Folding state checked by NMR.
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Biological Activity Comment
- > 80U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
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Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) (Active) protein (His tag)
MMP8 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-465) (Active) protein (His tag)MMP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 1-467) protein (GST tag)MMP8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) (AA 21-467) protein (His tag)MMP8 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8) proteinMMP8 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE
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Application Notes
- Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors
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Comment
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The enzyme was observed as a single band migrating at a molecular weight of < 20kDa
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Restrictions
- For Research Use only
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Buffer
- 0.2mg/ml in Tris 20mM pH 7.2, CaCl2 10mM, ZnCl2 0.1mM, NaCl 0.3M, Acetohydroxamic Acid (AHA) 0.5M.
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Storage
- -20 °C/-80 °C
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Storage Comment
- -80°C. The enzyme is stable at -20°C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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Alternative Name
- MMP-8
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Molecular Weight
- 17.7kDa
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UniProt
- P22894
Target
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