CD34 Protein (CD34)

Catalog No. ABIN1111306

This Recombinant CD34 protein is produced in CHO Cells.

Quick Overview for CD34 Protein (CD34) (ABIN1111306)

Target: CD34

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Biochemical Assay (BCA)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Purpose: Human CD34 Protein Recombinant

Characteristics:

Protein Size: Recombinant CD34 is a monomer protein consisting of 270 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human extracellular domain of human CD34 including a C-terminal 6His tag was expressed in HEK293 cells.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Measured by the ability to support the adhesion of the HUVEC cell line. Coated plates with immobilized recombinant Human CD34 ( 0.8 μg/mL) are able to adhere 40% of cells ( 4 x 104 cells/well) after one hour at 37 ℃

Application Details

Restrictions: For Research Use only

Handling

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

Buffer: Recombinant CD34 is supplied as a 0.2 μm filtered PBS solution, pH 7.2.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: CD34

Alternative Name: CD34

Molecular Weight: 50 kDa