HMGB1 Protein (BoxB Domain)
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- Target See all HMGB1 Proteins
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- BoxB Domain
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Origin
- Human, Mouse
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Source
- Escherichia coli (E. coli)
- Application
- Activation (Act), Cellular Assay (CA)
- Sequence
- MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV
- Characteristics
- BoxB recapitulates the cytokine activity of full length HMGB1 and efficiently activates macrophages to release tumor necrosis factor (TNF- ) and other proinflammatory cytokines (Li et al 2004). This product contains four amino acids (MARI) that have been added at the N terminus for the purpose of expression in bacteria. It contains only trace amounts of LPS (<0.4 ng/mg protein).
- Purification
- Purified
- Purity
- > 95 %
- Endotoxin Level
- The purified protein is free from LPS (Cambrex Limulus Amoebocyte Assay QCL-1000, <0.4 ng LPS per mg protein).
- Top Product
- Discover our top product HMGB1 Protein
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (Non-oxidizable) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: Hi-5 Cells Recombinant > 90 % by SDS - PAGE SDS
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
High Mobility Group Box 1 (HMGB1) (AA 2-215) protein (His tag)Crystallography grade HMGB1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The protein once resuspended can be stored frozen (-20°C).
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- Target
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Alternative Name
- High-mobility group protein B1 (HMGB1) (HMGB1 Products)
- Synonyms
- HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein
- Background
- BoxB is one of the DNA binding domains of HMGB1 protein. It consists of 90 amino acids and has a calculated molecular mass of approximately 10 kDa. The sequence of BoxB is totally identical in human and mouse.
- Molecular Weight
- approximately 10 kDa
- Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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