IL-6 Protein

Catalog No. ABIN1305111

This Recombinant IL-6 protein is expressed in Escherichia coli (E. coli) and has been mentioned in 4+ publications.

Quick Overview for IL-6 Protein (ABIN1305111)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Blocking Reagent (BR), Intracellular Flow Cytometry (ICFC)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity: Activity Range is measured in a proliferation assay using TF-1 indicator cells at 10^5 cells/ml
Specific Activity: 0.5 - 3.0 x 10^8 Unit /mg
ED50: 30- 200 pg/ml
Observed dose response: 50 fold.
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Characteristics: Recombinant human Interleukin-6 is supplied as a frozen liquid comprised of 0.22 µm filtered aqueous buffered solution, pH 7.2, 10% glycerol and containing 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Human IL-6 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant human IL-6 is supplied as a frozen liquid comprised of 0.22 μm filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-6 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of IL-6 as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human IL-6 is useful as a quantitative standard for measuring human IL-6 protein levels in an IL-6 specific sandwich ELISA with purified MQ2-13A5 antibody as a capture antibody (Cat. No. 554543) and the biotinylated MQ2-39C3 (Cat. No. 554546) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-6 standard from 2000-15 pg/ml should be included in each ELISA plate. To measure human IL-6 in plasma or serum please use our Human IL-6 BD OptEIA™ set, (Cat. No. 555220) or the Human IL-6 BD OptEIA™ Kit II (Cat. No. 550799).
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-6 can be used as a blocking control to demonstrate specificity of IL-6 staining by the FITC- or PE-MQ2-13A5 antibodies (Cat. No. 554544, 554545), or the FITC- or PE-MQ2-6A3 antibodies (Cat. No. 554696, 554697). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

Comment:

BD Pharmingen™ Recombinant Human IL-6 - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-6 (IL-6) is a potent lymphoid cell growth factor which affects B lymphocytes, T lymphocytes, and hybridoma cells. It will also affect cytotoxic T cells in combination with other factors, such as IL-2 and interferon-γ. Human IL-6 is a 20.5 kD protein containing 184 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Kitamura, Takaku, Miyajima: "IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1." in: International immunology, Vol. 3, Issue 6, pp. 571-7, (1991) (PubMed).

Van Snick: "Interleukin-6: an overview." in: Annual review of immunology, Vol. 8, pp. 253-78, (1990) (PubMed).

Hirano, Yasukawa, Harada, Taga, Watanabe, Matsuda, Kashiwamura, Nakajima, Koyama, Iwamatsu: "Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin." in: Nature, Vol. 324, Issue 6092, pp. 73-6, (1986) (PubMed).

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: Interleukin-6 (IL-6) is a potent lymphoid cell growth factor which affects B lymphocytes, T lymphocytes, and hybridoma cells. It will also affect cytotoxic T cells in combination with other factors, such as IL-2 and interferon-γ. Human IL-6 is a 20.5 kD protein containing 184 amino acid residues.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome