IL-3 Protein

Catalog No. ABIN1305115

Recombinant IL-3 protein expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for IL-3 Protein (ABIN1305115)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity: The activity range is measured in a proliferation assay using TF-1 cells.
Specific Activity: 0.3 - 1.4 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.)
ED50: 70-300 pg/ml
Observed dose response: > 200 fold

Characteristics: Recombinant human IL-3 is supplied as a frozen aqueous buffered solution containing glycerol, 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and with no preservatives. Recombinant human IL-3 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant human IL-3 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-3 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of human IL-3, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human IL-3 is useful as a quantitative standard for measuring human IL-3 protein levels in an IL-3 specific sandwich ELISA with the purified BVD8-3G11 antibody (Cat. No. 554672) as a capture antibody and the biotinylated BVD3-1F9 (Cat. No. 554674) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-3 standard from 2000 to 15 pg/ml should be included in each ELISA plate.
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: Recombinant human IL-3 can be used as a blocking control to demonstrate specificity of IL-3 staining by the directly conjugated-BVD3 1F9 antibody (Cat. No. 554676). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook both of which are posted on our web site.

Comment:

BD Pharmingen™ Recombinant Human IL-3 - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15 kD protein containing 133 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Frendl: "Interleukin 3: from colony-stimulating factor to pluripotent immunoregulatory cytokine." in: International journal of immunopharmacology, Vol. 14, Issue 3, pp. 421-30, (1992) (PubMed).

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Background: Interleukin-3 (IL-3) is a species specific colony stimulating factor which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Human IL-3 is a 15 kD protein containing 133 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy