PLK1 Protein (AA 1-603) (GST tag)

Catalog No. ABIN1315430

This Recombinant PLK1 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for PLK1 Protein (AA 1-603) (GST tag) (ABIN1315430)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-603

Purification tag / Conjugate: This PLK1 protein is labelled with GST tag.

Purpose: PLK1 (Human) Recombinant Protein (P01)

Sequence: MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR RRYVRGRFLG KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM EISIHRSLAH QHVVGFHGFF EDNDFVFVVL ELCRRRSLLE LHKRRKALTE PEARYYLRQI VLGCQYLHRN RVIHRDLKLG NLFLNEDLEV KIGDFGLATK VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP LPERPREKEE PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS LQYIERDGTE SYLTVSSHPN SLMKKITLLK YFRNYMSEHL LKAGANITPR EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK LILCPLMAAV TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL KAS

Characteristics: Human PLK1 full-length ORF ( AAH02369, 1 a.a. - 603 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Laurell, Beck, Krupina, Theerthagiri, Bodenmiller, Horvath, Aebersold, Antonin, Kutay: "Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry." in: Cell, Vol. 144, Issue 4, pp. 539-50, (2011) (PubMed).

Target Details

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1

Background: Full Gene Name: polo-like kinase 1 (Drosophila)
Synonyms: PLK,STPK13

Gene ID: 5347

Pathways: Cell Division Cycle, M Phase