PLK1 Protein (AA 1-603) (GST tag)
(1 reference)-
- Target See all PLK1 products
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-603
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PLK1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PLK1 (Human) Recombinant Protein (P01)
- Sequence
- MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR RRYVRGRFLG KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM EISIHRSLAH QHVVGFHGFF EDNDFVFVVL ELCRRRSLLE LHKRRKALTE PEARYYLRQI VLGCQYLHRN RVIHRDLKLG NLFLNEDLEV KIGDFGLATK VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP LPERPREKEE PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS LQYIERDGTE SYLTVSSHPN SLMKKITLLK YFRNYMSEHL LKAGANITPR EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK LILCPLMAAV TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL KAS
- Characteristics
- Human PLK1 full-length ORF ( AAH02369, 1 a.a. - 603 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Polo-Like Kinase 1 (PLK1) (AA 1-603) (Active) protein (GST tag)
PLK1 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Polo-Like Kinase 1 (PLK1) (AA 1-603) (Active) protein (His-GST)PLK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Polo-Like Kinase 1 (PLK1) (AA 501-603) protein (GST tag)PLK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polo-Like Kinase 1 (PLK1) (Active) protein (His tag)PLK1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE Active
Polo-Like Kinase 1 (PLK1) (Active) protein (His tag)PLK1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Polo-Like Kinase 1 (PLK1) (AA 2-603) protein (His tag)Crystallography grade PLK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polo-Like Kinase 1 (PLK1) (AA 2-603) protein (His tag)Crystallography grade PLK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polo-Like Kinase 1 (PLK1) protein (Myc-DYKDDDDK Tag)PLK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry." in: Cell, Vol. 144, Issue 4, pp. 539-50, (2011) (PubMed).
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: "Phosphorylation of Nup98 by multiple kinases is crucial for NPC disassembly during mitotic entry." in: Cell, Vol. 144, Issue 4, pp. 539-50, (2011) (PubMed).
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- Target
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Alternative Name
- PLK1 (PLK1 Products)
- Synonyms
- PLK Protein, STPK13 Protein, plk Protein, PLK-1 Protein, Plx1 Protein, stpk13 Protein, Plk Protein, cb525 Protein, cb636 Protein, ik:tdsubc_2d1 Protein, wu:fb37g11 Protein, wu:fb76g03 Protein, xx:tdsubc_2d1 Protein, polo like kinase 1 Protein, polo like kinase 1 S homeolog Protein, polo-like kinase 1 Protein, polo-like kinase 1 (Drosophila) Protein, Serine/threonine-protein kinase plk-1 Protein, PLK1 Protein, plk1.S Protein, plk1 Protein, Plk1 Protein, plk-1 Protein
- Background
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Full Gene Name: polo-like kinase 1 (Drosophila)
Synonyms: PLK,STPK13 - Gene ID
- 5347
- Pathways
- Cell Division Cycle, M Phase
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