PLK1 Protein (His tag)
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- Target See all PLK1 products
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PLK1 protein is labelled with His tag.
- Purpose
- Recombinant Mouse PLK1/PLK-1 Protein (His Tag)(Active)
- Sequence
- Met 1-Ser 603
- Characteristics
- A DNA sequence encoding the mouse PLK1 (Q07832) (Met 1-Ser 603) was fused with a polyhistidine tag at the N-terminus.
- Purity
- > 90 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 3 nmol/min/mg using casein as substrate.
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Polo-Like Kinase 1 (PLK1) (AA 1-603) protein (GST tag)
PLK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polo-Like Kinase 1 (PLK1) (AA 501-603) protein (GST tag)PLK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polo-Like Kinase 1 (PLK1) (Active) protein (His tag)PLK1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Polo-Like Kinase 1 (PLK1) protein (Myc-DYKDDDDK Tag)PLK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Alternative Name
- PLK1/PLK-1 (PLK1 Products)
- Synonyms
- PLK Protein, STPK13 Protein, plk Protein, PLK-1 Protein, Plx1 Protein, stpk13 Protein, Plk Protein, cb525 Protein, cb636 Protein, ik:tdsubc_2d1 Protein, wu:fb37g11 Protein, wu:fb76g03 Protein, xx:tdsubc_2d1 Protein, polo like kinase 1 Protein, polo like kinase 1 S homeolog Protein, polo-like kinase 1 Protein, polo-like kinase 1 (Drosophila) Protein, Serine/threonine-protein kinase plk-1 Protein, PLK1 Protein, plk1.S Protein, plk1 Protein, Plk1 Protein, plk-1 Protein
- Background
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Background: Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.
Synonym: Plk,STPK13
- Molecular Weight
- 70.6 kDa
- UniProt
- Q07832
- Pathways
- Cell Division Cycle, M Phase
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