Perforin 1 Protein (AA 22-555) (GST tag)

Catalog No. ABIN1316183

This Recombinant Perforin 1 protein is expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for Perforin 1 Protein (AA 22-555) (GST tag) (ABIN1316183)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)

Product Details

Protein Characteristics: AA 22-555

Purification tag / Conjugate: This Perforin 1 protein is labelled with GST tag.

Purpose: PRF1 (Human) Recombinant Protein (P01)

Sequence: PCHTAARSEC KRSHKFVPGA WLAGEGVDVT SLRRSGSFPV DTQRFLRPDG TCTLCENALQ EGTLQRLPLA LTNWRAQGSG CQRHVTRAKV SSTEAVARDA ARSIRNDWKV GLDVTPKPTS NVHVSVAGSH SQAANFAAQK THQDQYSFST DTVECRFYSF HVVHTPPLHP DFKRALGDLP HHFNASTQPA YLRLISNYGT HFIRAVELGG RISALTALRT CELALEGLTD NEVEDCLTVE AQVNIGIHGS ISAEAKACEE KKKKHKMTAS FHQTYRERHS EVVGGHHTSI NDLLFGIQAG PEQYSAWVNS LPGSPGLVDY TLEPLHVLLD SQDPRREALR RALSQYLTDR ARWRDCSRPC PPGRQKSPRD PCQCVCHGSA VTTQDCCPRQ RGLAQLEVTF IQAWGLWGDW FTATDAYVKL FLGGQELRTS TVWDNNNPIW SVRLDFGDVL LATGGPLRLQ VWDQDSGRDD DLLGTCDQAP KSGSHEVRCN LNHGHLKFRY HARCLPHLGG GTCLDYVPQM LLGEPPGNRS GAVW

Characteristics: Human PRF1 full-length ORF ( AAH63043.1, 22 a.a. - 555 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Suggested Applications*:elisa,antibody array,western blotting,affinity purification
*We do not guarantee this product working for suggested applications.
Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Lu, Lamontagne, Sun, Pinkerton, Block, Lu: "Role of the inflammatory protein serine protease inhibitor Kazal in preventing cytolytic granule granzyme A-mediated apoptosis." in: Immunology, Vol. 134, Issue 4, pp. 398-408, (2011) (PubMed).

Target Details

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: PRF1

Background: Full Gene Name: perforin 1 (pore forming protein)
Synonyms: FLH2,HPLH2,MGC65093,P1,PFN1,PFP

Gene ID: 5551

Pathways: Apoptosis, Caspase Cascade in Apoptosis