PYCARD Protein (AA 1-149) (GST tag)
(1 reference)Quick Overview for PYCARD Protein (AA 1-149) (GST tag) (ABIN1316968)
Target
See all PYCARD ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-149
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Purification tag / Conjugate
- This PYCARD protein is labelled with GST tag.
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Purpose
- PYCARD (Human) Recombinant Protein (P01)
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Sequence
- MDALDLTDKL VSFYLETYGA ELTANVLRDM GLQEMAGQLQ AATHQGSGAA PAGIQAPPQS AAKPGLHFID QHRAALIARV TNVEWLLDAL YGKVLTDEQY QAVRAEPTNP SKMRKLFNFT PAWNWTCKDL LLQALRESQS YLVEDLERS
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Characteristics
- Human PYCARD full-length ORF ( AAH13569.2, 1 a.a. - 149 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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PYD and CARD Domain Containing (PYCARD) (AA 1-195) protein (His tag)
custom-made PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (AA 1-193) protein (His tag)custom-made PYCARD Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).
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: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).
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- PYCARD (PYD and CARD Domain Containing (PYCARD))
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Alternative Name
- PYCARD
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Background
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Full Gene Name: PYD and CARD domain containing
Synonyms: ASC,CARD5,MGC10332,TMS,TMS-1,TMS1 -
Gene ID
- 29108
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Pathways
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
Target
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