SIRT1 Protein (AA 456-555) (GST tag)

Catalog No. ABIN1320039

Recombinant SIRT1 protein expressed in Wheat germ.

Quick Overview for SIRT1 Protein (AA 456-555) (GST tag) (ABIN1320039)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 456-555

Purification tag / Conjugate: This SIRT1 protein is labelled with GST tag.

Purpose: SIRT1 (Human) Recombinant Protein (Q01)

Sequence: NRYIFHGAEV YSDSEDDVLS SSSCGSNSDS GTCQSPSLEE PMEDESEIEE FYNGLEDEPD VPERAGGAGF GTDGDDQEAI NEAISVKQEV TDMNYPSNKS

Characteristics: Human SIRT1 partial ORF ( AAH12499, 456 a.a. - 555 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

Target Details

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: Full Gene Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Synonyms: SIR2L1

Gene ID: 23411

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling