SMAD3 Protein (AA 31-141) (GST tag)
Quick Overview for SMAD3 Protein (AA 31-141) (GST tag) (ABIN1320597)
Target
See all SMAD3 ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 31-141
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Purification tag / Conjugate
- This SMAD3 protein is labelled with GST tag.
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Purpose
- SMAD3 (Human) Recombinant Protein (P01)
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Sequence
- ELRLHHSFVL TGDVGRRICR LLVGLFTKGD TSSKRVHPFS PGPCFLLCDL ARVGSSPKIN VSPFYQNQTS TQRSCTVFVW QRCSLVGPFQ VTVFTMYFHH SLRSISRFSS G
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Characteristics
- Human SMAD3 full-length ORF ( AAH00414, 31 a.a. - 141 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)
SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) protein (GST tag,His tag)SMAD3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Alternative Name
- SMAD3
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Background
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Full Gene Name: SMAD family member 3
Synonyms: DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396 -
Gene ID
- 4088
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Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Target
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