SMAD3 Protein (DYKDDDDK Tag,His tag)
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- Target See all SMAD3 Proteins
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SMAD3 protein is labelled with DYKDDDDK Tag,His tag.
- Purpose
- Recombinant Human Mothers Against Decapentaplegic Homolog 3/SMAD3 (N-6His-Flag)
- Sequence
- MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product SMAD3 Protein
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)
SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 147-270) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) protein (GST tag,His tag)SMAD3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 2-425) protein (His tag)Crystallography grade SMAD3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 2-425) protein (His tag)Crystallography grade SMAD3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,500 mM NaCl, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Alternative Name
- Mothers Against Decapentaplegic Homolog 3/SMAD3 (SMAD3 Products)
- Synonyms
- HSPC193 Protein, HsT17436 Protein, JV15-2 Protein, LDS1C Protein, LDS3 Protein, MADH3 Protein, madh3 Protein, madh3a Protein, smad3 Protein, wu:fa99e03 Protein, XSmad3 Protein, XenMLP Protein, Xmad3 Protein, madh3-A Protein, Madh3 Protein, AU022421 Protein, Smad 3 Protein, mad3 Protein, madh3b Protein, zgc:92234 Protein, SMAD family member 3 Protein, SMAD family member 3a Protein, SMAD family member 3 L homeolog Protein, SMAD family member 3b Protein, SMAD3 Protein, smad3a Protein, smad3.L Protein, Smad3 Protein, smad3b Protein
- Molecular Weight
- 50.3 kDa
- UniProt
- P84022
- Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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