SMAD3 Protein (AA 1-425) (His tag)
Quick Overview for SMAD3 Protein (AA 1-425) (His tag) (ABIN666909)
Target
See all SMAD3 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-425
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Purification tag / Conjugate
- This SMAD3 protein is labelled with His tag.
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Sequence
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MSSILPFTPP IVKRLLGWKK GEQNGQEEKW CEKAVKSLVK KLKKTGQLDE LEKAITTQNV NTKCITIPRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELRAMELCE FAFNMKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEIPAEF PPLDDYSHSI PENTNFPAGI EPQSNIPETP PPGYLSEDGE TSDHQMNHSM DAGSPNLSPN PMSPAHNNLD LQPVTYCEPA FWCSISYYEL NQRVGETFHA SQPSMTVDGF TDPSNSERFC LGLLSNVNRN AAVELTRRHI GRGVRLYYIG GEVFAECLSD SAIFVQSPNC NQRYGWHPAT VCKIPPGCNL KIFNNQEFAA LLAQSVNQGF EAVYQLTRMC TIRMSFVKGW GAEYRRQTVT STPCWIELHL NGPLQWLDKV LTQMGSPSIR CSSVS -
Characteristics
- SMAD3, 1-425 aa, Human, His-tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)
SMAD3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)SMAD3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) protein (GST tag,His tag)SMAD3 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl and 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Alternative Name
- Smad3
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Background
- SMAD3, also known as mothers against decapentaplegic homolog 3, belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Small moleucule inhibitors of Smad3 may have tremendous clinical potential in the treatment of pathological fibrotic diseases. Recombinant SMAD3 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 50.2 kDa (445aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005893
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Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
Target
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