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TNF alpha Protein (AA 1-233) (GST tag)

Name: TNF alpha Protein (AA 1-233) (GST tag)
SKU: ABIN1323120
Price: 688.28 USD
Availability: InStock

This Recombinant TNF alpha protein is expressed in Wheat germ and has been mentioned in 3+ publications.

Image no. 1 for Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (GST tag) (ABIN1323120) (TNF alpha Protein (AA 1-233) (GST tag))

1 image

(3 references)

Catalog No. ABIN1323120

Quick Overview for TNF alpha Protein (AA 1-233) (GST tag) (ABIN1323120)

Target

See all TNF alpha Proteins

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type

Recombinant

Origin

Human

Source

Wheat germ

Application

Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Discover our top product TNF alpha Protein

Protein Characteristics

AA 1-233

Purification tag / Conjugate

This TNF alpha protein is labelled with GST tag.

Purpose

TNF (Human) Recombinant Protein (P01)

Sequence

MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL

Characteristics

Human TNF full-length ORF ( AAH28148, 1 a.a. - 233 a.a.) recombinant protein with GST-tag at N-terminal.

Purification

in vitro wheat germ expression system
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Product

Expression System

Conjugate

Origin

Price starts at

TNF alpha Protein (AA 1-233) (His tag)

Expression System HEK-293 Cells

Conjugate His tag

Origin Human

Price starts at $16,231.82

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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)
custom-made TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)
custom-made TNF alpha Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein
HPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Tumor Necrosis Factor alpha (TNF alpha) (Active) protein
TNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active

Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions

For Research Use only
Buffer

50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-80 °C

Storage Comment

Best use within three months from the date of receipt of this protein.
Kwak, Han, Bae: "Aspalathin and nothofagin from rooibos (Aspalathus linearis) inhibit endothelial protein C receptor shedding in vitro and in vivo." in: Fitoterapia, Vol. 100, pp. 179-86, (2015) (PubMed).

Ku, Kim, Bae: "Piperlonguminine downregulates endothelial protein C receptor shedding in vitro and in vivo." in: Inflammation, Vol. 37, Issue 2, pp. 435-42, (2014) (PubMed).

Ku, Kim, Han, Bae: "Antithrombotic activities of epi-sesamin in vitro and in vivo." in: The American journal of Chinese medicine, Vol. 41, Issue 6, pp. 1313-27, (2013) (PubMed).
Target

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name

TNF

Background

Full Gene Name: tumor necrosis factor (TNF superfamily, member 2)
Synonyms: DIF,TNF-alpha,TNFA,TNFSF2

Gene ID

7124

Pathways

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome