FNDC5 Protein (AA 29-140, AA 32-143)

Catalog No. ABIN1343991

This Recombinant FNDC5 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FNDC5 Protein (AA 29-140, AA 32-143) (ABIN1343991)

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Protein Type: Recombinant

Origin: Human, Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 29-140, AA 32-143

Purpose: Irisin (rec.) (untagged) (E.coli)

Cross-Reactivity: Human, Mouse

Characteristics: Irisin (human, aa 32-143 / mouse, aa 29-140) is untagged. Irisin has 100 % identity between mouse, rat, monkey and human.

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: Lyophilized from PBS or TRIS buffer (lot-dependent).

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))

Alternative Name: Irisin

Background:

Fibronectin Type III Domain-containing Protein 5 (cleaved), Fibronectin Type III Repeat-containing Protein 2 (cleaved), FNDC5 (cleaved)

Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone Irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure.

Molecular Weight: ~12kDa

UniProt: Q8NAU1

Pathways: Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation