Calreticulin Protein (CALR) (His tag)

Catalog No. ABIN1344123

This Recombinant Calreticulin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Calreticulin Protein (CALR) (His tag) (ABIN1344123)

Target: Calreticulin (CALR)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Purification tag / Conjugate: This Calreticulin protein is labelled with His tag.

Purpose: Calreticulin (human) (rec.) (His)

Cross-Reactivity: Human

Characteristics: Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Calreticulin (CALR)

Alternative Name: Calreticulin

Background:

CRP55, Calregulin, Endoplasmic Reticulum Resident Protein 60, ERp60, grp60

Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the 'calreticulin/calnexin cycle' that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.

Molecular Weight: ~55kDa (SDS-PAGE)

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response