CD40 Ligand Protein (CD40LG) (AA 18-111, AA 115-260) (DYKDDDDK Tag)

Catalog No. ABIN1344285

This Recombinant CD40 Ligand protein is expressed in CHO Cells and has been mentioned in 2+ publications.

Quick Overview for CD40 Ligand Protein (CD40LG) (AA 18-111, AA 115-260) (DYKDDDDK Tag) (ABIN1344285)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Mouse

Source: CHO Cells

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 115-260, AA 18-111

Purification tag / Conjugate: This CD40 Ligand protein is labelled with DYKDDDDK Tag.

Purpose: CD40L (mouse) (multimeric) (rec.)

Specificity: Binds to human and mouse CD40.

Cross-Reactivity: Human, Mouse

Characteristics: Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Guarda, Dostert, Staehli, Cabalzar, Castillo, Tardivel, Schneider, Tschopp: "T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes." in: Nature, Vol. 460, Issue 7252, pp. 269-73, (2009) (PubMed).

Holler, Tardivel, Kovacsovics-Bankowski, Hertig, Gaide, Martinon, Tinel, Deperthes, Calderara, Schulthess, Engel, Schneider, Tschopp: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L

Background:

MultimericCD40L™, ACRP30headless:CD40L, ACRP30headless:CD154, ACRP30headless:TNFSF5, ADIPOQ-CD40L

The costimulatory molecule CD40, a member of the tumor necrosis factor (TNF) receptor superfamily, critically regulates B cell and T cell function in adaptive immunity and inflammation by interacting with CD40L (CD154). CD40L mediates a range of activities on B cells, including induction of activation-associated surface antigen, entry into cell cycle, isotype switching, immunoglobulin secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and DC maturation. MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced. MultimericCD40L™ enhances B cell proliferation.

Molecular Weight: ~35-40kDa

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints