TRAIL Protein (AA 95-281, Extracellular Domain, N-Term, Soluble)

Catalog No. ABIN1344417

Recombinant TRAIL protein expressed in Escherichia coli (E. coli).

Quick Overview for TRAIL Protein (AA 95-281, Extracellular Domain, N-Term, Soluble) (ABIN1344417)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 95-281, Extracellular Domain, N-Term, Soluble

Purpose: izTRAIL, Soluble (human) (rec.)

Specificity: Binds to human TRAIL receptors 1-4 (TRAIL-R1 to TRAIL-R4), izTRAIL does not interact with the apoptosis-inducing mouse TRAIL receptor (TRAIL-R).

Characteristics: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to an isoleucine zipper motif.

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment: Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1μg/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500μg per day (Wissink 2006).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1μg/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500μg per day (Wissink 2006).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.1 mg/mL after reconstitution

Concentration: Lot specific

Buffer: Contains 20 mM TRIS-Cl, 0.5M arginine-HCl, 100 mM NaCl, 0.02 % Tween 20.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Background:

Alternate Names/Synonyms: Apo-2L, TNFSF10, CD253

Product Description: izTRAIL is a newly available, highly active recombinant form of soluble human TRAIL. Due to a trimerizing N-terminal isoleucine zipper (iz) motif the intrinsic trimerization of TRAIL, required for apoptosis-inducing activity of TRAIL, is enhanced when compared to non-tagged soluble human TRAIL (shTRAIL). Therefore, izTRAIL is a potent inducer of apoptosis in many human cancer cells, but not normal human hepatocytes. In addition, the half-life of izTRAIL is about eight-fold higher than the half-life of shTRAIL. These properties render izTRAIL highly suitable for both, in vitro and in vivo use, particularly for studies in which investigators plan to transfer their in vitro results into an in vivo system with human cancer cells in xenotransplant settings examining susceptibility to TRAIL-induced apoptosis.

Molecular Weight: ~82kDa as stable trimers (determined by size exclusion chromatography) ~28kDa as monomer (determined by SDS-PAGE)

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity