ARAF Protein (AA 1-609) (GST tag)
Quick Overview for ARAF Protein (AA 1-609) (GST tag) (ABIN1345432)
Target
See all ARAF ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 1-609
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Purification tag / Conjugate
- This ARAF protein is labelled with GST tag.
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Purpose
- ARAF (Human) Recombinant Protein (P02)
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Sequence
- MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RQQPSRFYHS VQDLSGGSRQ HEAPSNRPLN ELLTPQGPSP RTQHCDPEHF PFPAPANAPL QRIRSTSTPN VHMVSTTAPM DSNLIQLTGQ SFSTDAAGSR GGSDGTPRGS PSPASVSSGR KSPHSKSPAE QRERKSLADD KKKVKNLGYR DSGYYWEVPP SEVQLLKRIG TGSFGTVFRG RWHGDVAVKV LKVSQPTAEQ AQAFKNEMQV LRKTRHVNIL LFMGFMTRPG FAIITQWCEG SSLYHHLHVA DTRFDMVQLI DVARQTAQGM DYLHAKNIIH RDLKSNNIFL HEGLTVKIGD FGLATVKTRW SGAQPLEQPS GSVLWMAAEV IRMQDPNPYS FQSDVYAYGV VLYELMTGSL PYSHIGCRDQ IIFMVGRGYL SPDLSKISSN CPKAMRRLLS DCLKFQREER PLFPQILATI ELLQRSLPKI ERSASEPSLH RTQADELPAC LLSAARLVP
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Characteristics
- Human ARAF full-length ORF ( AAH07514, 1 a.a. - 609 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-604) protein (His tag)
custom-made ARAF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) protein (His tag)custom-made ARAF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) protein (GST tag)ARAF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP, KAA
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-227) protein (His-IF2DI Tag)ARAF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) protein (Myc-DYKDDDDK Tag)ARAF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
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Alternative Name
- ARAF
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Background
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Gene: v-raf murine sarcoma 3611 viral oncogene homolog
Synonyms: A-RAF, ARAF1, PKS2, RAFA1 -
Gene ID
- 369
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Pathways
- MAPK Signaling, RTK Signaling, Hepatitis C
Target
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