CYR61 Protein (AA 1-381) (GST tag)

Catalog No. ABIN1351026

This Recombinant CYR61 protein is expressed in Wheat germ and has been mentioned in 16+ publications.

Quick Overview for CYR61 Protein (AA 1-381) (GST tag) (ABIN1351026)

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Product Details

Protein Characteristics: AA 1-381

Purification tag / Conjugate: This CYR61 protein is labelled with GST tag.

Purpose: CYR61 (Human) Recombinant Protein (P01)

Sequence: MSSRIARALA LVVTLLHLTR LAPSTCPAAC HCPLEAPKCA PGVGLVRDGC GCCKVCAKQL NEDCSKTQPC DHTKGLECNF GASSTALKGI CRAQSEGRPC EYNSRIYQNG ESFQPNCKHQ CTCIDGAVGC IPLCPQELSL PNLGCPNPRL VKVTGQCCEE WVCDEDSIKD PMEDQDGLLG KELGFDASEV ELTRNNELIA VGKGSSLKRL PVFGMEPRIL YNPLQGQKCI VQTTSWSQCS KTCGTGISTR VTNDNPECRL VKETRICKVR PCGQPVYSSL KKGKKCSKTK KSPEPVRFTY AGCLSVKKYR PKYCGSCVDG RCCTPQLTRT VKMRFRCEDG ETFSKNVMMI QSCKCNYNCP HANEAAFPFY RLFNDIHKFR D

Characteristics: Human CYR61 full-length ORF ( AAH01271.1, 1 a.a. - 381 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Jeansonne, Pacifici, Lassak, Reiss, Russo, Zabaleta, Peruzzi: "Differential Effects of MicroRNAs on Glioblastoma Growth and Migration." in: Genes, Vol. 4, Issue 1, pp. 46-64, (2014) (PubMed).

Kok, Lin, Hou, Hong, Chang, Hsiao, Wang, Lai, Lin: "Simvastatin inhibits cysteine-rich protein 61 expression in rheumatoid arthritis synovial fibroblasts through the regulation of sirtuin-1/FoxO3a signaling." in: Arthritis and rheumatism, Vol. 65, Issue 3, pp. 639-49, (2013) (PubMed).

You, Yang, Yang, Chen: "Cyr61 induces the expression of monocyte chemoattractant protein-1 via the integrin ???3, FAK, PI3K/Akt, and NF-?B pathways in retinal vascular endothelial cells." in: Cellular signalling, Vol. 26, Issue 1, pp. 133-40, (2013) (PubMed).

Terada, Shiraishi, Zeng, Mooney, Yeater, Mangold, Partin, Kulkarni, Getzenberg: "Cyr61 is regulated by cAMP-dependent protein kinase with serum levels correlating with prostate cancer aggressiveness." in: The Prostate, Vol. 72, Issue 9, pp. 966-76, (2012) (PubMed).

Wu, Lin, Lee, Kok, Wang, Hou, Yang, Lai, Wang, Hong: "Epigallocatechin-3-gallate diminishes cytokine-stimulated Cyr61 expression in human osteoblastic cells: a therapeutic potential for arthritis." in: Rheumatology (Oxford, England), Vol. 51, Issue 11, pp. 1953-65, (2012) (PubMed).

Kok, Hou, Hong, Wang, Liang, Chang, Hsiao, Yang, Lai, Lin: "Simvastatin inhibits cytokine-stimulated Cyr61 expression in osteoblastic cells: a therapeutic benefit for arthritis." in: Arthritis and rheumatism, Vol. 63, Issue 4, pp. 1010-20, (2011) (PubMed).

Bär, Dorfman, Fischer, Hilfiker-Kleiner, Cebotari, Tudorache, Suprunov, Haverich, Hilfiker: "The pro-angiogenic factor CCN1 enhances the re-endothelialization of biological vascularized matrices in vitro." in: Cardiovascular research, Vol. 85, Issue 4, pp. 806-13, (2010) (PubMed).

Su, Chiou, Tang, Zhao, Tsai, Chen, Chang, Chien, Peng, Hsiao, Kuo, Yen: "CYR61 regulates BMP-2-dependent osteoblast differentiation through the {alpha}v{beta}3 integrin/integrin-linked kinase/ERK pathway." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31325-36, (2010) (PubMed).

You, Yang, Chen, Yang: "Regulation of Cyr61/CCN1 expression by hypoxia through cooperation of c-Jun/AP-1 and HIF-1? in retinal vascular endothelial cells." in: Experimental eye research, Vol. 91, Issue 6, pp. 825-36, (2010) (PubMed).

Chai, Norng, Modak, Reavis, Mouazzen, Pham: "CCN1 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 8, pp. 1140-51, (2010) (PubMed).

You, Yang, Chen, Yang: "Cysteine-rich 61, a member of the CCN family, as a factor involved in the pathogenesis of proliferative diabetic retinopathy." in: Investigative ophthalmology & visual science, Vol. 50, Issue 7, pp. 3447-55, (2009) (PubMed).

Lin, Kuo, Chang, Chu, Chen, Lin, Sureshbabu, Shih, Kuo: "Involvement of hypoxia-inducing factor-1alpha-dependent plasminogen activator inhibitor-1 up-regulation in Cyr61/CCN1-induced gastric cancer cell invasion." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15807-15, (2008) (PubMed).

Grote, Salguero, Ballmaier, Dangers, Drexler, Schieffer: "The angiogenic factor CCN1 promotes adhesion and migration of circulating CD34+ progenitor cells: potential role in angiogenesis and endothelial regeneration." in: Blood, Vol. 110, Issue 3, pp. 877-85, (2007) (PubMed).

Athanasopoulos, Schneider, Keiper, Alt, Pendurthi, Liegibel, Sommer, Nawroth, Kasperk, Chavakis: "Vascular endothelial growth factor (VEGF)-induced up-regulation of CCN1 in osteoblasts mediates proangiogenic activities in endothelial cells and promotes fracture healing." in: The Journal of biological chemistry, Vol. 282, Issue 37, pp. 26746-53, (2007) (PubMed).

Lin, Chang, Chen, Wu, Wang, Kuo, Chu, Chang, Lee, Chen, Kuo, Lin: "Cysteine-rich 61 (CCN1) enhances chemotactic migration, transendothelial cell migration, and intravasation by concomitantly up-regulating chemokine receptor 1 and 2." in: Molecular cancer research : MCR, Vol. 5, Issue 11, pp. 1111-23, (2007) (PubMed).

OConnor, Mills, Jones, Ho, Pavlath: "A combinatorial role for NFAT5 in both myoblast migration and differentiation during skeletal muscle myogenesis." in: Journal of cell science, Vol. 120, Issue Pt 1, pp. 149-59, (2006) (PubMed).

Target Details

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Alternative Name: CYR61

Background: Full Gene Name: cysteine-rich, angiogenic inducer, 61
Synonyms: CCN1,GIG1,IGFBP10

Gene ID: 3491

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding