CYR61 Protein (AA 25-381)

Catalog No. ABIN7866012

This Recombinant CYR61 protein is produced in Escherichia coli (E. coli).

Quick Overview for CYR61 Protein (AA 25-381) (ABIN7866012)

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 25-381

Purpose: Human CYR61 Protein Recombinant

Sequence: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF

Characteristics:

Protein Size: Recombinant CYR61 migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human CYR61 mature chain was expressed in E. Coli. Recombinant Human Protein CYR61 encodes amino acid residues 25-381.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to inhibit IGF-II induced proliferation of MCF-7

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: CYR61 was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Alternative Name: CYR61

Molecular Weight: 25 kDa

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding