Granulin Protein (GRN) (AA 494-593) (GST tag)
(3 references)-
- Target See all Granulin (GRN) Proteins
- Granulin (GRN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 494-593
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Granulin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- GRN (Human) Recombinant Protein (Q01)
- Sequence
- SCEKEVVSAQ PATFLARSPH VAVKDVECGE GHFCHDNQTC CRDNRQGWAC CPYRQGVCCA DRRHCCPAGF RCAARGTKCL RREAPRWDAP LRDPALRQLL
- Characteristics
- Human GRN partial ORF ( NP_002078, 494 a.a. - 593 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Granulin (GRN) (AA 364-430) protein (His tag)
GRN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Granulin (GRN) (AA 289-426) protein (His-IF2DI Tag)GRN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Granulin (GRN) (AA 1-593) protein (GST tag)GRN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Granulin (GRN) protein (His tag)GRN Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Granulin (GRN) (AA 18-593) protein (His tag)Crystallography grade GRN Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Granulin (GRN) (AA 18-589) protein (His tag)Crystallography grade GRN Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 21, pp. 9202-13, (2013) (PubMed).
: "The neurotrophic properties of progranulin depend on the granulin E domain but do not require sortilin binding." in: Neurobiology of aging, Vol. 34, Issue 11, pp. 2541-7, (2013) (PubMed).
: "Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival." in: The Journal of cell biology, Vol. 181, Issue 1, pp. 37-41, (2008) (PubMed).
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: "Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 21, pp. 9202-13, (2013) (PubMed).
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- Target
- Granulin (GRN)
- Alternative Name
- GRN (GRN Products)
- Synonyms
- GRN Protein, grn Protein, wu:fb11g12 Protein, wu:fj18f05 Protein, MGC53354 Protein, DKFZp459H066 Protein, CLN11 Protein, GEP Protein, GP88 Protein, PCDGF Protein, PEPI Protein, PGRN Protein, epithelin Protein, acrogranin Protein, granulin precursor Protein, granulin a Protein, Granulins Protein, granulin L homeolog Protein, granulin Protein, GRN Protein, grna Protein, grn Protein, grn.L Protein, granulin Protein, ORF1 granulin Protein, Grn Protein
- Background
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Full Gene Name: granulin
Synonyms: GEP,GP88,PCDGF,PEPI,PGRN - Gene ID
- 2896
- NCBI Accession
- NM_002087
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