IL-3 Protein

Catalog No. ABIN1589649

This Recombinant IL-3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-3 Protein (ABIN1589649)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >98 % by SDS-PAGE

Product Details

Purpose: Recombinant Human IL-3 Protein

Sequence: MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

Specificity: Chromosomal location:5q31.1

Characteristics: Length (aa):134

Endotoxin Level: < 0.1 ng/μg of protein (< 1EU/μg)

Application Details

Application Notes: The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1 - 0.5 ng/mL. The WHO standard #91/510 was used as a control.

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: water,The lyophilized IL-3 should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: PBS

Storage: 0 °C,-20 °C

Storage Comment: The lyophilized IL-3, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-3 should be stored in working aliquots at -20°C.

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Background:

Synonyms: IL3, IL-3, MCGF, MULTI-CSF

Description: IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human IL-3 is a 15.0 kDa globular protein containing 133 amino acid residues.

Molecular Weight: 15.0 kDa

Gene ID: 3562

NCBI Accession: NM_000588, NP_000579

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy