VEGFC Protein

Catalog No. ABIN1589785

Recombinant VEGFC protein expressed in Insect Cells.

Quick Overview for VEGFC Protein (ABIN1589785)

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Insect Cells

Purity: > 90 % by SDS-PAGE

Product Details

Purpose: VEGF-C

Sequence: DTVKLAAAHY NTEILKSIDN EWRKTQCMPR EVCIDVGKEF GAATNTFFKP PCVSVYRCGG CCNSEGLQCM NTSTGYLSKT LFEITVPLSQ GPKPVTISFA NHTSCRCMSK LDVYRQVHSI IHHHHHH

Specificity: Chromosomal location:16p11

Characteristics: Length (aa):127

Application Details

Application Notes: The biological activity was determined (i) by the ability to induce VEGFR-3/FLT-4 receptor phosphorylation in PAEC/VEGFR-3 cells and (ii) the VEGF-C-induced proliferation of primary human dermal lymphatic endothelial cells (HDLEC).

Comment:

Cytokines & Growth Factors

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized VEGF-C is soluble in water and most aqueous buffers. The lyophilized VEGF-C should be reconstituted in PBS or medium to a concentration not lower than 50 μg/mL.

Buffer: 50 mM acetic acid

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Expiry Date: 6 months

Target Details

Target: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Alternative Name: VEGF-C

Background: VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 127 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 15-20 kDa protein in SDS-PAGE under reducing conditions.
Synonyms: vascular endothelial growth factor C, Vegfc

Molecular Weight: 15.0 - 20.0 kDa

Gene ID: 114111

NCBI Accession: NM_053653, NP_446105

UniProt: O35757

Pathways: RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2