ERK1 Protein (AA 1-370) (His tag)

Catalog No. ABIN7585049

Recombinant ERK1 protein expressed in Yeast.

Quick Overview for ERK1 Protein (AA 1-370) (His tag) (ABIN7585049)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Protein Type: Recombinant

Origin: Arabidopsis thaliana

Source: Yeast

Application: ELISA

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-370

Purification tag / Conjugate: This ERK1 protein is labelled with His tag.

Sequence: MNTGGGQYTD FPAVETHGGQ FISYDIFGSL FEITSKYRPP IIPIGRGAYG IVCSVLDTET NELVAMKKIA NAFDNHMDAK RTLREIKLLR HLDHENIIAI RDVVPPPLRR QFSDVYISTE LMDTDLHQII RSNQSLSEEH CQYFLYQLLR GLKYIHSANI IHRDLKPSNL LLNANCDLKI CDFGLARPTS ENDFMTEYVV TRWYRAPELL LNSSDYTAAI DVWSVGCIFM ELMNRKPLFP GKDHVHQMRL LTELLGTPTE SDLGFTHNED AKRYIRQLPN FPRQPLAKLF SHVNPMAIDL VDRMLTFDPN RRITVEQALN HQYLAKLHDP NDEPICQKPF SFEFEQQPLD EEQIKEMIYQ EAIALNPTYG

Specificity: Arabidopsis thaliana (Mouse-ear cress)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: Mitogen-activated protein kinase 3 (MPK3)

Background: Recommended name: Mitogen-activated protein kinase 3.
Short name= AtMPK3.
Short name= MAP kinase 3.
EC= 2.7.11.24

UniProt: Q39023

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins