Poliovirus Receptor Protein (PVR) (C-Term, Extracellular Domain)

Catalog No. ABIN1684657

This Recombinant Poliovirus Receptor protein is produced in HEK-293T Cells.

Quick Overview for Poliovirus Receptor Protein (PVR) (C-Term, Extracellular Domain) (ABIN1684657)

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293T Cells

Purity: > 95 %, as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: C-Term, Extracellular Domain

Specificity: Optimized DNA sequence encoding extracellular domain of Mouse PVR CD155 including a C-terminal 6His tag was expressed in HEK293 cells.

Characteristics: Recombinant mouse PVR (CD155) is a monomer protein consisting of 329 amino acid residue subunits, due to glycosylation migrates as an approximately 55-60 kDa protein on SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Application Details

Comment:

The biological activity of Mouse PVR was measured by the binding ability of immobilized recombinant Mouse CD155 (1 µg/mL100 µL/well ) to mouse CD226 (linear range of 1 - 100 g/mL) in a functional ELISA assay.

Restrictions: For Research Use only

Handling

Buffer: PBS solution, pH7.2

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Expiry Date: 12-24 months

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155

Background: M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T-cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto-oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of5 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.

UniProt: Q8K094

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome