GOT1 Protein (AA 2-414) (His tag)
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- Target See all GOT1 Proteins
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-414
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GOT1 protein is labelled with His tag.
- Purpose
- Recombinant Human Glutamate Oxaloacetate Transaminase 1/GOT1 (C-6His)
- Sequence
- APPSVFAEVP QAQPVLVFKL TADFREDPDP RKVNLGVGAY RTDDCHPWVL PVVKKVEQKI ANDNSLNHEY LPILGLAEFR SCASRLALGD DSPALKEKRV GGVQSLGGTG ALRIGADFLA RWYNGTNNKN TPVYVSSPTW ENHNAVFSAA GFKDIRSYRY WDAEKRGLDL QGFLNDLENA PEFSIVVLHA CAHNPTGIDP TPEQWKQIAS VMKHRFLFPF FDSAYQGFAS GNLERDAWAI RYFVSEGFEF FCAQSFSKNF GLYNERVGNL TVVGKEPESI LQVLSQMEKI VRITWSNPPA QGARIVASTL SNPELFEEWT GNVKTMADRI LTMRSELRAR LEALKTPGTW NHITDQIGMF SFTGLNPKQV EYLVNEKHIY LLPSGRINVS GLTTKNLDYV ATSIHEAVTK IQLEHHHHHH
- Characteristics
- Recombinant Human Glutamate Oxaloacetate Transaminase 1/GOT1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Leu414) of Human GOT1.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product GOT1 Protein
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Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 1-413) protein (GST tag)
GOT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)GOT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1) (AA 2-413) protein (His tag)GOT1 Origin: Human Host: Yeast (Pichia pastoris) Recombinant > 95 % SDS, WB, ELISA, IP
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 2 mM DTT, 20 % Glycerol, pH 7.5 .
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))
- Alternative Name
- Glutamate Oxaloacetate Transaminase 1/GOT1 (GOT1 Products)
- Background
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Glutamate Oxaloacetate Transaminase 1 (GOT1) is a cytoplasmic protein. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT1 is a pyridoxal phosphate-dependent enzyme that exists in cytoplasmic and mitochondrial forms. GOT1 plays a key role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT1 involves in L-methionine salvage from methylthioadenosine, aspartate catabolic process, cellular response to insulin stimulus, polyamine metabolic process, and glucocorticoid stimulus.
Alternative Names: Aspartate Aminotransferase Cytoplasmic, Glutamate Oxaloacetate Transaminase 1, Transaminase A, GOT1 - Molecular Weight
- 47.31 kDa
- UniProt
- P17174
- Pathways
- Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process
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