PPM1G Protein (AA 317-546) (His tag)

Catalog No. ABIN1690901

This Recombinant PPM1G protein is produced in Escherichia coli (E. coli).

Quick Overview for PPM1G Protein (AA 317-546) (His tag) (ABIN1690901)

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 317-546

Purification tag / Conjugate: This PPM1G protein is labelled with His tag.

Purpose: Recombinant Human Protein Phosphatase 1G/PP1MG (C-6His)

Sequence: MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD LEHHHHHH

Characteristics: Recombinant Human Protein Phosphatase 1G/PP1MG is produced with our E. coli expression system. The target protein is expressed with sequence (Met317-Asp546) of Human PPM1G.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 25 mM TrisHCl, 1 mM DTT, 1 mM EDTA, 2 mM ß-ME, 20 % Glycerol, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))

Alternative Name: ppm1g

Background: Protein Phosphatase 1G (PP1MG) is a cytoplasmic protein that belongs to the PP2C family. PPM1G is widely expressed, it is abundant in testis, skeletal muscle, and heart. PPM1G is a negative regulator of cell stress response pathways. PPM1G is responsible for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G also plays a role in regulating cell cycle progression.
Alternative Names: Protein Phosphatase 1G, Protein Phosphatase 1C, Protein Phosphatase 2C Isoform Gamma, PP2C-Gamma, Protein Phosphatase Magnesium-Dependent 1 Gamma, PPM1G, PPM1C

Molecular Weight: 26.15 kDa

UniProt: O15355