PPM1G Protein (AA 1-546) (His tag)
-
- Target See all PPM1G Proteins
- PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-546
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PPM1G protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
- Purity
- > 85 % by SDS-PAGE
- Top Product
- Discover our top product PPM1G Protein
-
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 1-546) protein (GST tag)
PPM1G Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 462-544) protein (GST tag)PPM1G Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (AA 1-546) protein (His tag)PPM1G Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PPM1G Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 1) protein (His tag)PPM1G Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PPM1G Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl , 0.1 mM PMSF
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- PPM1G (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G (PPM1G))
- Alternative Name
- PPM1G (PPM1G Products)
- Synonyms
- AI385652 Protein, Fin13 Protein, PP2CG Protein, PP2CGAMMA Protein, PPP2CG Protein, protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform Protein, protein phosphatase, Mg2+/Mn2+ dependent 1G Protein, protein phosphatase, Mg2+/Mn2+ dependent, 1G Protein, Ppm1g Protein, PPM1G Protein
- Background
- PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 61.4 kDa (566aa)
- NCBI Accession
- NP_817092
- UniProt
- O15355
-
