PSPH Protein (AA 1-225) (His tag)

Catalog No. ABIN1690908

This Recombinant PSPH protein is produced in Escherichia coli (E. coli).

Quick Overview for PSPH Protein (AA 1-225) (His tag) (ABIN1690908)

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-225

Purification tag / Conjugate: This PSPH protein is labelled with His tag.

Purpose: Recombinant Human Phosphoserine Phosphatase/PSP (C-6His)

Sequence: MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEELEHHH HHH

Characteristics: Recombinant Human Phosphoserine Phosphatase is produced by our E.coli expression system and the target gene encoding Met1-Glu225 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM Tris,4M Urea,5 mM EDTA, pH 8.0.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Alternative Name: PSPase

Background: Alternative Names: Phosphoserine Phosphatase, PSP, PSPase, L-3-Phosphoserine Phosphatase, O-Phosphoserine Phosphohydrolase, PSPHPhosphoserine phosphatase (PSP) is an enzyme that belongs to the serB family. PSPH catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. Deficiency of this protein is thought to be linked to Williams syndrome. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome.
Alternative Names: Phosphoserine Phosphatase, PSP, PSPase, L-3-Phosphoserine Phosphatase, O-Phosphoserine Phosphohydrolase, PSPH

Molecular Weight: 26.07 kDa

UniProt: P78330

Pathways: Warburg Effect