OTUB2 Protein (AA 1-234) (GST tag)
Quick Overview for OTUB2 Protein (AA 1-234) (GST tag) (ABIN1690983)
Target
See all OTUB2 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-234
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Purification tag / Conjugate
- This OTUB2 protein is labelled with GST tag.
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Purpose
- Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 (N-GST)
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Sequence
- MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSE TSFNLISEKC DILSILRDHP ENRIYRRKIE ELSKRFTAIR KTKGDGNCFY RALGYSYLES LLGKSREIFK FKERVLQTPN DLLAAGFEEH KFRNFFNAFY SVVELVEKDG SVSSLLKVFN DQSASDHIVQ FLRLLTSAFI RNRADFFRHF IDEEMDIKDF CTHEVEPMAT ECDHIQITAL SQALSIALQV EYVDEMDTAL NHHVFPEAAT PSVYLLYKTS HYNILYAADK H
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Characteristics
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Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 (N-GST)
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2) (AA 1-234) protein (His tag)
custom-made OTUB2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2) (AA 1-234) protein (His tag)custom-made OTUB2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2) (AA 1-234) protein (His tag)OTUB2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2) (AA 1-234) protein (GST tag)OTUB2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5 .
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- OTUB2 (OTU Domain, Ubiquitin Aldehyde Binding 2 (OTUB2))
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Alternative Name
- Ubiquitin Thioesterase OTUB2/OTUB2
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Background
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Recombinant Human Ubiquitin Thioesterase OTUB2/OTUB2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-His234) of Human OTUB2 fused with a GST tag at the N-terminus.
Ubiquitin Thioesterase OTUB2 belongs to the peptidase C65 family, functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro, may plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB2 is expressed at higher levels in the brain. -
Molecular Weight
- 53.6 kDa
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UniProt
- Q96DC9
Target
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