SGSH Protein (AA 21-502) (His tag)

Catalog No. ABIN1691379

This Recombinant SGSH protein is produced in Human Cells.

Quick Overview for SGSH Protein (AA 21-502) (His tag) (ABIN1691379)

Target: SGSH (N-Sulfoglucosamine Sulfohydrolase (SGSH))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-502

Purification tag / Conjugate: This SGSH protein is labelled with His tag.

Purpose: Recombinant Human N-Sulphoglucosamine Sulphohydrolase/SGSH (C-6His)

Sequence: RPRNALLLLA DDGGFESGAY NNSAIATPHL DALARRSLLF RNAFTSVSSC SPSRASLLTG LPQHQNGMYG LHQDVHHFNS FDKVRSLPLL LSQAGVRTGI IGKKHVGPET VYPFDFAYTE ENGSVLQVGR NITRIKLLVR KFLQTQDDRP FFLYVAFHDP HRCGHSQPQY GTFCEKFGNG ESGMGRIPDW TPQAYDPLDV LVPYFVPNTP AARADLAAQY TTVGRMDQGV GLVLQELRDA GVLNDTLVIF TSDNGIPFPS GRTNLYWPGT AEPLLVSSPE HPKRWGQVSE AYVSLLDLTP TILDWFSIPY PSYAIFGSKT IHLTGRSLLP ALEAEPLWAT VFGSQSHHEV TMSYPMRSVQ HRHFRLVHNL NFKMPFPIDQ DFYVSPTFQD LLNRTTAGQP TGWYKDLRHY YYRARWELYD RSRDPHETQN LATDPRFAQL LEMLRDQLAK WQWETHDPWV CAPDGVLEEK LSPQCQPLHN ELVDHHHHHH

Characteristics: Recombinant Human N-Sulphoglucosamine Sulphohydrolase/SGSH is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Arg21-Leu502) of Human SGSH fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM GaCl2,10 % Glycerol, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SGSH (N-Sulfoglucosamine Sulfohydrolase (SGSH))

Sub Type: Fusionprotein

Background: N-Sulphoglucosamine Sulphohydrolase (SGSH) is an important member of the sulfatase family which is involved in the degradation of heparin sulfate. SGSH binds one calcium ion per subunit as a cofactor. SGSH catalyzes N-sulfo-D-glucosamine and H2O to D-glucosamine and sulfate. SGSH deficiency is result in mucopolysaccharidosis type 3A (MPS3A), a recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations.
Alternative Names: N-Sulphoglucosamine Sulphohydrolase, Sulfoglucosamine Sulfamidase, Sulphamidase, SGSH, HSS

Molecular Weight: 55.72 kDa

UniProt: P51688

Pathways: Glycosaminoglycan Metabolic Process