PAI1 Protein (AA 24-402) (His tag)
Quick Overview for PAI1 Protein (AA 24-402) (His tag) (ABIN1691566)
Target
See all PAI1 (SERPINE1) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 24-402
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Purification tag / Conjugate
- This PAI1 protein is labelled with His tag.
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Purpose
- Recombinant Mouse Plasminogen Activator Inhibitor 1/PAI-1/SERPIN E1 (C-6His)
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Sequence
- LPLRESHTAH QATDFGVKVF QQVVQASKDR NVVFSPYGVS SVLAMLQMTT AGKTRRQIQD AMGFKVNEKG TAHALRQLSK ELMGPWNKNE ISTADAIFVQ RDLELVQGFM PHFFKLFQTM VKQVDFSEVE RARFIINDWV ERHTKGMISD LLAKGAVDEL TRLVLVNALY FSGQWKTPFL EASTHQRLFH KSDGSTVSVP MMAQSNKFNY TEFTTPDGLE YDVVELPYQG DTLSMFIAAP FEKDVHLSAL TNILDAELIR QWKGNMTRLP RLLILPKFSL ETEVDLRGPL EKLGMPDMFS ATLADFTSLS DQEQLSVAQA LQKVRIEVNE SGTVASSSTA FVISARMAPT EMVIDRSFLF VVRHNPTETI LFMGQVMEPV DHHHHHH
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Characteristics
- Recombinant Mouse Plasminogen activator inhibitor 1/SERPIN E1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu24-Ser402) of Mouse SERPIN E1 fused with a polyhistidine tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 1-402) protein (GST tag)
SERPINE1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)SERPINE1 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 86-185) protein (GST tag)SERPINE1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Plasminogen Activator Inhibitor 1 (SERPINE1) protein (His tag)SERPINE1 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)SERPINE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Plasminogen Activator Inhibitor 1 (SERPINE1) (AA 24-402) protein (His tag)SERPINE1 Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. -
Expiry Date
- 5 months
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- PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
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Alternative Name
- SERPIN E1
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Sub Type
- Fusionprotein
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Background
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Plasminogen activator inhibitor-1 (serpin E1) is a serine protease inhibitor which belongs to the serpin family. Serpin E1 acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Alternative Names: Plasminogen activator inhibitor 1, Endothelial plasminogen activator inhibitor, Serpin E1, Mr1, Pai1, Planh1, -
Molecular Weight
- 43.7 kDa
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UniProt
- P22777
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Pathways
- p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
Target
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