Survivin Protein (AA 1-142)

Catalog No. ABIN1691881

This Recombinant Survivin protein is produced in Escherichia coli (E. coli).

Quick Overview for Survivin Protein (AA 1-142) (ABIN1691881)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-142

Purpose: Recombinant Human Baculoviral IAP Repeat-Containing Protein 5/BIRC5/Survivin

Sequence: MGAPTLPPAW QPFLKDHRIS TFKNWPFLEG CACTPERMAE AGFIHCPTEN EPDLAQCFFC FKELEGWEPD DDPIEEHKKH SSGCAFLSVK KQFEELTLGE FLKLDRERAK NKIAKETNNK KKEFEETAKK VRRAIEQLAA MD

Characteristics: Recombinant Human Baculoviral IAP Repeat-Containing Protein 5/BIRC5 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp142) of Human BIRC5.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 0.1M NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: BIRC5

Background: Baculoviral IAP Repeat-Containing Protein 5 (BIRC5) belongs to the IAP family. BIRC5 exists as a homodimer in the apo state and as a monomer in the CPC-bound state. BIRC5 contains one BIR repeat and is expressed only in fetal kidney and liver, and to lesser extent, lung and brain. BIRC5 functions as a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. BIRC5 is also a component of a chromosome passage protein complex (CPC), which is essential for chromosome alignment and segregation during mitosis and cytokinesis. BIRC5 acts as an important regulator of the localization of this complex.
Alternative Names: Baculoviral IAP Repeat-Containing Protein 5, Apoptosis Inhibitor 4, Apoptosis Inhibitor Survivin, BIRC5, API4, IAP4

Molecular Weight: 20.4 kDa

UniProt: O15392

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding