DCK Protein (AA 1-260) (His tag)
Quick Overview for DCK Protein (AA 1-260) (His tag) (ABIN667827)
Target
See all DCK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-260
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Purification tag / Conjugate
- This DCK protein is labelled with His tag.
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Sequence
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MATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL -
Characteristics
- DCK, 1-260aa, Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)
custom-made DCK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxycytidine Kinase (DCK) (AA 1-260) protein (His tag)custom-made DCK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deoxycytidine Kinase (DCK) (AA 1-260) protein (GST tag)DCK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2 mM EDTA, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DCK (Deoxycytidine Kinase (DCK))
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Alternative Name
- DCK
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Background
- DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 34.6 kDa (296aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_000779
Target
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