STAT1 Protein (AA 1-712)

Catalog No. ABIN1691998

Recombinant STAT1 protein expressed in Escherichia coli (E. coli).

Quick Overview for STAT1 Protein (AA 1-712) (ABIN1691998)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-712

Purpose: Recombinant Human Signal Transducer and Activator of Transcription 1/STAT1

Sequence: GSHMSQWYEL QQLDSKFLEQ VHQLYDDSFP MEIRQYLAQW LEKQDWEHAA NDVSFATIRF HDLLSQLDDQ YSRFSLENNF LLQHNIRKSK RNLQDNFQED PIQMSMIIYS CLKEERKILE NAQRFNQAQS GNIQSTVMLD KQKELDSKVR NVKDKVMCIE HEIKSLEDLQ DEYDFKCKTL QNREHETNGV AKSDQKQEQL LLKKMYLMLD NKRKEVVHKI IELLNVTELT QNALINDELV EWKRRQQSAC IGGPPNACLD QLQNWFTIVA ESLQQVRQQL KKLEELEQKY TYEHDPITKN KQVLWDRTFS LFQQLIQSSF VVERQPCMPT HPQRPLVLKT GVQFTVKLRL LVKLQELNYN LKVKVLFDKD VNERNTVKGF RKFNILGTHT KVMNMEESTN GSLAAEFRHL QLKEQKNAGT RTNEGPLIVT EELHSLSFET QLCQPGLVID LETTSLPVVV ISNVSQLPSG WASILWYNML VAEPRNLSFF LTPPCARWAQ LSEVLSWQFS SVTKRGLNVD QLNMLGEKLL GPNASPDGLI PWTRFCKENI NDKNFPFWLW IESILELIKK HLLPLWNDGC IMGFISKERE RALLKDQQPG TFLLRFSESS REGAITFTWV ERSQNGGEPD FHAVEPYTKK ELSAVTFPDI IRNYKVMAAE NIPENPLKYL YPNIDKDHAF GKYYSRPKEA PEPMELDGPK GTGYIKTELI SVSEV

Characteristics:

Recombinant Human Signal Transducer and Activator of Transcription 1/STAT1

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: Signal Transducer and Activator of Transcription 1-alpha/beta/stat1

Background: Recombinant Human Signal Transducer and Activator of Transcription 1-α/β/STAT1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val712) of Human STAT1.
Signal Transducer and Activator of Transcription 1-α/β (STAT1) contains one SH2 domain and belongs to the transcription factor STAT family. When tyrosine- and serine-phosphorylated, STAT1 can form a homodimer termed IFN-γ-activated factor (GAF), migrate into the nucleus and bind to the IFN γ activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. STAT1 functions as signal transducer and transcription activator that mediates cellular responses to interferons. Defects in STAT1 are the cause of STAT1 deficiency complete and familial candidiasis type 7.

Molecular Weight: 83.3 kDa

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events