P4HB Protein (AA 18-505) (His tag)

Catalog No. ABIN1692112

This Recombinant P4HB protein is produced in Escherichia coli (E. coli).

Quick Overview for P4HB Protein (AA 18-505) (His tag) (ABIN1692112)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 18-505

Purification tag / Conjugate: This P4HB protein is labelled with His tag.

Purpose: Recombinant Human Prolyl 4-Hydroxylase Subunit β/P4HB/PDIA1 (C-6His)

Sequence: DAPEEEDHVL VLRKSNFAEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA GKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFRN GDTASPKEYT AGREADDIVN WLKKRTGPAA TTLPDGAAAE SLVESSEVAV IGFFKDVESD SAKQFLQAAE AIDDIPFGIT SNSDVFSKYQ LDKDGVVLFK KFDEGRNNFE GEVTKENLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSNFKT AAESFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESEELTAE RITEFCHRFL EGKIKPHLMS QELPEDWDKQ PVKVLVGKNF EDVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIVIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDG AGDDDDLEDL EEAEEPDMEE DDDQKAVKVD HHHHHH

Characteristics:

Recombinant Human Prolyl 4-Hydroxylase Subunit β/P4HB/PDIA1 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: Prolyl 4-Hydroxylase Subunit beta/p4hb

Background: Recombinant Human Prolyl 4-Hydroxylase Subunit β/P4HB is produced with our E. coli expression system. The target protein is expressed with sequence (Asp18-Lys505) of Human P4HB fused with a 6His tag at the C-terminus.
Protein Disulfide-Isomerase (P4HB) is an endoplasmic reticulum lumen protein that belongs to the protein disulfide isomerase family. P4HB contains two thioredoxin domains and catalyzes the formation, breakage, and rearrangement of -S-S- bonds in proteins. P4HB is involved in hydroxylation of prolyl residues in preprocollagen. P4HB has the ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner. P4HB plays a role in both the influx and efflux of S-nitrosothiol-bound nitric oxide.

Molecular Weight: 55.9 kDa

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism