P4HB Protein (AA 1-508) (GST tag)
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- Target See all P4HB Proteins
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-508
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This P4HB protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- P4HB (Human) Recombinant Protein (P01)
- Sequence
- MLRRALLCLA VAALVRADAP EEEDHVLVLR KSNFAEALAA HKYLLVEFYA PWCGHCKALA PEYAKAAGKL KAEGSEIRLA KVDATEESDL AQQYGVRGYP TIKFFRNGDT ASPKEYTAGR EADDIVNWLK KRTGPAATTL PDGAAAESLV ESSEVAVIGF FKDVESDSAK QFLQAAEAID DIPFGITSNS DVFSKYQLDK DGVVLFKKFD EGRNNFEGEV TKENLLDFIK HNQLPLVIEF TEQTAPKIFG GEIKTHILLF LPKSVSDYDG KLSNFKTAAE SFKGKILFIF IDSDHTDNQR ILEFFGLKKE ECPAVRLITL EEEMTKYKPE SEELTAERIT EFCHRFLEGK IKPHLMSQEL PEDWDKQPVK VLVGKNFEDV AFDEKKNVFV EFYAPWCGHC KQLAPIWDKL GETYKDHENI VIAKMDSTAN EVEAVKVHSF PTLKFFPASA DRTVIDYNGE RTLDGFKKFL ESGGQDGAGD DDDLEDLEEA EEPDMEEDDD QKAVKDEL
- Characteristics
- Human P4HB full-length ORF ( NP_000909.2, 1 a.a. - 508 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 18-508) protein (His tag)
P4HB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (Active) protein (His tag)P4HB Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 18-508) protein (His tag)Crystallography grade P4HB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Alternative Name
- P4HB (P4HB Products)
- Synonyms
- DSI Protein, ERBA2L Protein, GIT Protein, P4Hbeta Protein, PDI Protein, PDIA1 Protein, PHDB Protein, PO4DB Protein, PO4HB Protein, PROHB Protein, PDA2 Protein, PDIP Protein, PDIR Protein, ERp59 Protein, Pdia1 Protein, Thbp Protein, p55 Protein, XPDIp Protein, pdi Protein, 1810041F13Rik Protein, AI661267 Protein, Pdip Protein, Pdipl Protein, p58 Protein, prolyl 4-hydroxylase subunit beta Protein, protein disulfide isomerase family A member 2 Protein, prolyl 4-hydroxylase, beta polypeptide Protein, protein disulfide isomerase family A member 2 L homeolog Protein, protein disulfide isomerase associated 2 Protein, prolyl 4-hydroxylase subunit beta L homeolog Protein, protein disulfide-isomerase Protein, P4HB Protein, PDIA2 Protein, P4hb Protein, pdia2.L Protein, Pdia2 Protein, p4hb.L Protein, LOC8281065 Protein
- Background
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Full Gene Name: prolyl 4-hydroxylase, beta polypeptide
Synonyms: DSI,ERBA2L,GIT,P4Hbeta,PDI,PDIA1,PHDB,PO4DB,PO4HB,PROHB - Gene ID
- 5034
- NCBI Accession
- NM_000918
- Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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