UBE2K Protein (AA 1-200) (His-SUMO Tag)

Catalog No. ABIN1692388

This Recombinant UBE2K protein is produced in Escherichia coli (E. coli).

Quick Overview for UBE2K Protein (AA 1-200) (His-SUMO Tag) (ABIN1692388)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-200

Purification tag / Conjugate: This UBE2K protein is labelled with His-SUMO Tag.

Purpose: Recombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K (N-6His, SUMO tag)

Sequence: MGHHHHHHGS DSEVNQEAKP EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQAPEDLD MEDNDIIEAH REQIGGMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 K is produced by our E.coli expression system and the target gene encoding Met1-Asn200 is expressed with a 6His, SUMO tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: Ubiquitin-Conjugating Enzyme E2 K/UBE2K

Background: Alternative Names: Ubiquitin-Conjugating Enzyme E2 K, Huntingtin-Interacting Protein 2, HIP-2, Ubiquitin Carrier Protein, Ubiquitin-Conjugating Enzyme E2-25 kDa, Ubiquitin-Conjugating Enzyme E2(25K), Ubiquitin-Conjugating Enzyme E2-25K, Ubiquitin-Protein Ligase, UBE2K, HIP2, LIGRecombinant Human Ubiquitin-Conjugating Enzyme E2 K/UBE2K is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn200) of Human UBE2K fused with a 6His tag at the N-terminus.
Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain, with highest levels found in cortex and striatum, and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with huntingtin, and MHC-heavy chain proteins.

Molecular Weight: 34.5 kDa

UniProt: P61086