UBE2K Protein (GST tag)

Catalog No. ABIN7319151

Product Details

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This UBE2K protein is labelled with GST tag.

Purpose: Recombinant Human HIP2/UBE2K Protein (GST Tag)

Sequence: Met 1-Asn200

Characteristics: Recombinant Human Ubiquitin-Conjugating Enzyme E2 K is produced by our E.coli expression system and the target gene encoding Met1-Asn200 is expressed with a GST tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10 % Glycerol, pH 7.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: HIP2/UBE2K (UBE2K Products)

Synonyms: E2-25K Protein, HIP2 Protein, HYPG Protein, LIG Protein, UBC1 Protein, E2-25k Protein, Hip2 Protein, Hypg Protein, Lig Protein, e2-25k Protein, hip2 Protein, hypg Protein, lig Protein, AW492011 Protein, D5Ertd601e Protein, ube2k Protein, zgc:103472 Protein, zgc:110791 Protein, ubiquitin conjugating enzyme E2 K Protein, ubiquitin-conjugating enzyme E2K Protein, ubiquitin conjugating enzyme E2 K S homeolog Protein, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) Protein, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) Protein, UBE2K Protein, Ube2k Protein, ube2k.S Protein, ube2k Protein, ube2kb Protein, ube2ka Protein

Background:

Background: Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain, with highest levels found in cortex and striatum, and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with huntingtin, and MHC-heavy chain proteins.

Synonym: Ubiquitin-Conjugating Enzyme E2 K, Huntingtin-Interacting Protein 2, HIP-2, Ubiquitin Carrier Protein, Ubiquitin-Conjugating Enzyme E2-25 kDa, Ubiquitin-Conjugating Enzyme E2(25K), Ubiquitin-Conjugating Enzyme E2-25K, Ubiquitin-Protein Ligase, UBE2K, HIP2, LIG

Molecular Weight: 48.7 kDa

UniProt: P61086