UBE2D3 Protein (AA 1-147)
Quick Overview for UBE2D3 Protein (AA 1-147) (ABIN1880587)
Target
See all UBE2D3 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-147
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Purpose
- Recombinant Human Ubiquitin-Conjugating Enzyme E2 D3/UBE2D3
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Sequence
- MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PELARIYKTD RDKYNRISRE WTQKYAM
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Characteristics
- Recombinant Human Ubiquitin-conjugating enzyme E2 D3/UBE2D3 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of Human UBE2D3.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (His tag)
custom-made UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (GST tag)UBE2D3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-149) protein (His tag)UBE2D3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147), (full length) protein (His-SUMO Tag)UBE2D3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 9) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT,10 % glycerol, pH 7.5.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- -80 °C
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Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Expiry Date
- 6 months
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- UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))
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Alternative Name
- Ubiquitin-Conjugating Enzyme E2 D3/UBE2D3
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Background
- UBE2D3 is an enzyme that belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.
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Molecular Weight
- 16.6 kDa
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UniProt
- P61077
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Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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