IFNW1 Protein

Catalog No. ABIN2017994

Product Details

Target: IFNW1 (IFNW) (Interferon, omega 1 (IFNW))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: CDLPQNHGLL SRNTLVLLHQ MRRISPFLCL KDRRDFRFPQ EMVKGSQLQK AHVMSVLHEM LQQIFSLFHT ERSSAAWNMT LLDQLHTGLH QQLQHLETCL LQVVGEGESA GAISSPALTL RRYFQGIRVY LKEKKYSDCA WEVVRMEIMK SLFLSTNMQE RLRSKDRDLG SS

Characteristics: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human TF-1 cells is less than 0.01 ng/mL, corresponding to a specific activity of > 1.0 x 10^8 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1 EU/μg of rHuIFN-μ as determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.

Target Details

Target: IFNW1 (IFNW) (Interferon, omega 1 (IFNW))

Alternative Name: Interferon omega (IFNW Products)

Synonyms: IFNW1 Protein, interferon omega 1 Protein, interferon, omega 1 Protein, inteferon omega 1 Protein, IFNW1 Protein, LOC100155760 Protein, IFN-OMEGA1 Protein

Background: Interferon-Omega (IFN-o) coded by IFNW1 gene in human, is a number of the type I interferon family, which includes IFN-a, IFN-b, and IFN-o. The IFNAR-1/IFNAR-2 receptor complex can help with the signal transduction, followed the antiviral or the antiproliferative actions. IFN-omega is derived from IFN-a/b and share 75 % sequence with IFN-a. It has two intramolecular disulfide bonds which are crucial for activities. Mire-Sluis et al have described bioassays for IFN-a, IFN-b, and IFN-o that exploit the ability of these factors to inhibit proliferation of TF-1 cells induced by GM-CSF. The bioassays can be used also with Epo and TF-1 cells, or Epo and Epo-transfected UT-7 cells.
Synonyms: IFN Omega Human

Molecular Weight: 20.0 kDa, containing 172 amino acid residues with two conserved disulfide bonds.